Biosíntesis, caracterización y evaluación de la respuesta antiproliferativa de nanopartículas de plata, obtenidas a base de extractos de annona muricata, sobre líneas celulares de cáncer de mama.

Abstract

ABSTRACT Currently treatments for breast cancer are expensive and not very accessible, due to this, it requires the development of new routes of administration, treatments and novel materials, such as the one proposed. Carrying out the biosynthesis of silver nanoparticles (AgNPs) using extracts of a plant well known for its anticancer activity known as "Soursop" (Annona muricata); where the main active compounds of A. muricata (in leaves, stems and husk) are acetogenins and polyphenols, among others, which means that they can be used as reducing agents; It should be noted that anticancer activity is directly attributed to acetogenins. The biosynthesized AgNPs with leaf extract (AgNPs-EH) and with shell extract (AgNPs-EC) of A. muricata; were characterized by means of the UV-Vis technique, to observe the optical properties of the AgNPs, comparing the reaction times against the growth of the optical response known as Surface Plasmonic Resonance (RPS), as well as Transmission Electron Microscopy (TEM), to recognize the morphology and the average sizes of nanoparticle, with the help of the Scanning Electron Microscopy (SEM), a chemical mapping was achieved, to observe the distribution of the AgNPs in the medium, as well as with the help of the EDS technique (Energy-dispersive X-ray spectroscopy) the elemental chemical composition of the AgNPs was identified. In addition, by means of FT-IR it was possible to identify the vibrational mode of the functional groups present in the extracts. Achieving elucidate the cell viability of AgNPs in cell lines such as MCF-7, MDA-MB-468 and macrophages