The relationship between cyto-genotoxic damage and oxidative stress produced by emerging pollutants on a bioindicator organism (Allium cepa): The carbamazepine case.

Abstract

he carbamazepine (CBZ) is one of the most frequently detected anticonvulsant drugs in water bodies. Although there are reports of its ecotoxicological effects in the scienti!c literature, toxicity studies have not focused on establishing the mechanism by which CBZ produces its effect at environmentally relevant concentrations. The objective of this work was to evaluate cyto-genotoxicity and its relationship with oxidative stress produced by carbamazepine in the Allium cepa model. The cytotoxicity and genotoxicity, as well as the biomarkers of oxidative stress were analyzed in the roots of A. cepa, exposed to 1 and 31.36 mg L"1 after 2, 6, 12, 24, 48 and 72 h. The results show that genotoxic capacity of this drug in the roots of A. cepa is related to the generation of oxidative stress, in particular with production of hydro- peroxides and oxidized proteins. Also, the cytotoxic effect has a high correlation with DNA damage. The results of the present study clearly indicate that bioassays with sensitive plants such as A. cepa are useful and complementary tools to evaluate the environmental impact of emerging contaminants.