Expression of AdipoR1 and AdipoR2 Receptors as Leptin-Breast Cancer regulation mechanisms

Abstract

The development of breast cancer is influenced by the adipose tissue through the proteins leptin and adiponectin. However, there is little research concerning AdipoR1 and AdipoR2 receptors and the influence of leptin over them. The objective of this work was to analyze the expression of AdipoR1 and AdipoR2, modulated by differential concentrations of leptin in an obesity model (10ng/mL, 100ng/mL, and 1000ng/mL) associated with breast cancer in MCF-7 and HCC1937 cell lines. Each cell was characterized through immunohistochemistry, and the expression of AdipoR1 and AdipoR2 was analyzed by PCR in real time using TaqMan® probes. Leptin induced an increase in cell population of MCF-7 (23.8%, 10ng/m, 48 h) and HCC1937 (17.24%, 1000ng/mL, 72 h). In MCF-7, the expression of AdipoR1 decreased (3.81%, 1000ng/Ml) and the expression of AdipoR2 increased by 13.74 times (10ng/mL) with regard to the control. In HCC1937, the expression of AdipoR1 decreases by 86.28% (10ng/mL), as well as the expression of AdipoR1 and AdipoR2 mRNA.