In vitro inhibition of Botrytis cinerea with extracts of wild grape (Vitis spp.) leaves

Abstract

The extracts of wild grapevines (Vitis) leaves (EHVS) from three accessions (P178, E-200 and TN-4) at 6, 8 and 12 % v/v, were tested in vitro to evaluate their inhibitory effect on Botrytis cinerea development. Resveratrol (RVS) (60, 90, 120 µg/mL), citrus extracts (EC) (3, 5 and 8 % v/v) and cyprodinil + fludioxonil (SW) (500, 800 and 1000 µg/mL) were compared against EHVS. In average, SW presented inhibition of mycelial growth (ICM), sporulation (IE) and spore germination (IGC) (88.9; 85.5 and 93.7 %, average respectively). RVS presented the second higher inhibition rate. All EHVS presented antifungal activity; specially, P-178 at 12 % resulted in 72 % ICM, 75 % IE and 62 % IGC. This dose contained phenolic compounds 19.9 mg/mL, RVS 1.7 mg/mL, gallic acid 3.8 mg/mL and ferulic acid 2.5 mg/mL.