Chronic consumption of sweeteners increases carbonylated protein production in lymphocytes from mouse lymphoid organs

Abstract

Background: The prevalence of overweight, obesity and diabetes mellitus has increased in Mexico, therefore, sucralose and stevia are being used as alternative non-caloric sweeteners to reduce energy intake. Moreover, poorly balanced diets can lead to the formation of carbonyl groups, a marker used to determine oxidative damage to proteins. Increased presence of carbonylated proteins in CD1 mice chronically consuming sweeteners, may point them as causing oxidative damage. Aims: To determine whether the continued use of natural and artificial sweeteners increases the presence of carbonylated proteins in lymphocytes of lymphoid tissues in CD1 male mice. Methods: The present study was conducted with 72 CD1 newly weaned (21-day old) male mice, fed with standard lab diet and water ad libitum; mice were hosted in cages in groups of 4 under controlled temperature conditions (19-21°C), and light/dark cycles of 12/12 h. Weight and food intake was quantified weekly. Three groups of mice were randomly conformed: a) Baseline (21-day old, newly weaned, n=8); b) 6-week of treatment (63-day old, n=32); c) 12-week of treatment (105-day old, n=32). Groups b and c were divided into 4 subgroups each (n=8): i) Control (CL) without sweeteners; ii) Sucrose (SUC); iii) Sucralose (SUCL), and iv) Stevia (ST). Body weight, food, and water consumption were measured, and BMI was calculated from those values. Lymphocytes from Peyer's patches, peripheral blood and spleen were isolated, and from these cells carbonylated protein concentration was quantified. Blood glucose was also assessed. Results: Mice in SUCL and ST groups had lower weight gain and BMI compared to those that consumed SUC. The SUCL group consumed more food and the ST group decreased food intake, as compared with SUC and control groups. ST group drank more sweetened water, compared to the other groups. The percentage of blood lymphocytes and the carbonylated proteins concentrations were higher in the SUCL group. Conclusions: The chronic consumption of sucralose, caused an increase in food intake. In addition, the percentage of lymphocytes circulating in blood was elevated, as well as the concentration of carbonylated proteins in these cells.