Resumen:
Despite the widespread use of nanotechnology in radio-imaging applications, lipoprotein based delivery
systems have received only limited attention so far. These studies involve the synthesis of a novel hydrophobic
radio-imaging tracer consisting of a hydrazinonicotinic acid (HYNIC)-N-dodecylamide and 99mTc
conjugate that can be encapsulated into rHDL nanoparticles (NPs). These rHDL NPs can selectively target
the Scavenger Receptor type B1 (SR-B1) that is overexpressed on most cancer cells due to excess
demand for cholesterol for membrane biogenesis and thus can target tumors in vivo. We provide details
of the tracer synthesis, characterization of the rHDL/tracer complex, in vitro uptake, stability studies and
in vivo application of this new radio-imaging approach